Ascorbate synthesis-dependent glutathione consumption in mouse liver
نویسندگان
چکیده
منابع مشابه
Age-associated perturbations in glutathione synthesis in mouse liver.
The nature of the mechanisms underlying the age-related decline in glutathione (GSH) synthetic capacity is at present unclear. Steady-state kinetic parameters of mouse liver GCL (glutamate-cysteine ligase), the rate-limiting enzyme in GSH synthesis, and levels of hepatic GSH synthesis precursors from the trans-sulfuration pathway, such as homocysteine, cystathionine and cysteine, were compared ...
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Three major forms of cytosolic glutathione S-transferase (designated F1, F2, and F3 transferases according to increasing isoelectric points) were purified to homogeneity from liver of DBA/2J mice, primarily by CM-cellulose and hydroxylapatite chromatography. The purified enzymes were shown to have specific activities of 104, 281, and 143 units/mg, respectively, when assayed with 1 m~ each of l-...
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The mechanism which is responsible for the formation of individual peptide bonds in biological systems has so far remained obscure. There has as yet been no successful demonstration of an in vitro enzymatic synthesis of a simple peptide composed of natural amino acids. Various investigators have approached this problem by employing model substances which contain a CO-NH linkage but differ from ...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1996
ISSN: 0014-5793
DOI: 10.1016/0014-5793(96)00077-4